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doi: 10.3389/fnut.2026.1779855 Received 02 January 2026 Revised 02 February 2026 Accepted 19 February 2026 Published 04 March 2026 Volume 13 - 2026 Edited by Karolina Wojtunik-Kulesza, Medical University of Lublin, Poland Reviewed by Changhao He, Zhejiang Chinese Medical University, China Adriano Dos Santos, University of Bern, Switzerland Updates Copyright © 2026 Pinaffi-Langley, Zuraikat, St-Onge and Yabluchanskiy

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Results Disruption of the glucagon receptor significantly alters both gene expression and the interactive network of α cells To comprehensively examine the functional and physiological changes in α cells following GCGR blockade, we conducted single-cell RNA sequencing (scRNA-seq) on islets isolated from 12–14-week-old male wild-type (WT) and GCGR-knockout (KO) mice (Fig