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Western blot analysis and Coomassie Brilliant Blue (CBB) staining Samples were boiled in sample loading buffer at 100 °C for 10 min, separated by SDS-PAGE, and transferred onto NC membranes for blotting
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Furthermore, we harvested primary hepatocytes from Btg2 -/- mice in vitro and performed adenoviral knockdown of Fmo1 (Fig
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