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Ki-67 can distinguish quiescent G0 cells from cycling cells, EdU or BrdU incorporation marks active DNA synthesis in S phase, and phospho-histone H3 staining identifies mitotic cells within the 4N population
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Future research should focus on large-scale, well-controlled randomized trials with standardized dosing protocols, clearly defined deficiency thresholds, and long-term follow-up to strengthen the evidence base and guide clinical practice
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