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The list of differentially expressed genes was uploaded to Ingenuity Pathway Analysis (QIAGEN IPA), and core analysis yielded a list of canonical pathways, of which the top 10 upregulated and downregulated pathways were visualized on bar plots using ggplot v3.5.0 [29]
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There are multiple mechanisms that are involved in activating Nrf2, including interaction with cysteine residues on Keap1, disruption of Nrf2/Keap1 interaction, epigenetic modification and activation of protein kinases
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