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To minimize such unwanted events, we developed a standardized thawing procedure for frozen samples, with a small sample volume (150-200 µL): thawing under strict cooling conditions (+0°C to +2°C) within 12-15 minutes in an ice-water bath, without agitation

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Human studies from the 1980s and 1990s gave it by intravenous infusion to people with chronic insomnia and to healthy volunteers, with mixed sleep results