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Acute retinal ischemia sparks a critical depletion of ATP and glutamine synthetase [162,163,164], resulting in elevated levels of extracellular glutamate that trigger a destructive excitotoxicity cascade characterized by neuron depolarization, the influx of Ca 2+ ions, oxidative stress, and robust glutamatergic stimulation, which contributes to the degenerative repercussions of ischemia and reperfusion scenarios [165]

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Outcome measures cover histological damage scoring, cytokine panels (TNF-α, IL-6, IL-10), mucosal blood flow, tight junction protein expression by Western blot, and stool consistency scoring for colitis models