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Although such a mechanism has been demonstrated in the context of CD8⁺ T cells affecting GBM cell viability, direct investigations involving CSE -deficient GBM cells and NK cell interactions could provide mechanistic validation for the findings observed in CRISPR-based screening studies
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A Vanquish Horizon UHPLC system coupled to a Q Exactive Plus MS (both Thermo Fisher Scientific) was used to detect glutarate or succinate
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