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How acetyl-CoA-dependent enzymes interact with other acyl-CoAs is also emerging as another potentially important node of metabolic regulation

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Recipes YPD medium (1 L) 10 g of Yeast extract dried 20 g of hipolypeptone 20 g of D-(+)-glucose 1.0 ml of aureobasidin A stock solution (if needed) 20 g of agar powder (if needed) Ultrapure water Aureobasidin A stock solution (1 L) 0.5 mg of aureobasidin A 99.5% ethanol 500 mM EDTA (1 L) 186.12 g of EDTA disodium salt dihydrate Ultrapure water Adjust pH to 8.0 with 1.0 M NaOH 100 mM EDTA (1 L) 200 ml of 500 mM EDTA Ultrapure water HPLC mobile phase (1 L) 6.12 g of KH 2 PO 4 1.818 g of 1-heptanesulfonate 212.6 ml of methanol Ultrapure water Adjust pH to 2.8 with 98% phosphoric acid Acknowledgments This work was supported by a Special Coordination Fund for Promoting Science and Technology, Creation of Innovative Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction, Kobe) from the Ministry of Education, Culture, Sports, and Science and Technology (MEXT), Japan